Purified inotodiol inhibited HeLa cell proliferation and induced apoptosis via cyclin E and p27 rather than via cyclin D1 and CDK4.
What the researchers found
Purified inotodiol inhibited HeLa cervical cancer cell proliferation dose-dependently and induced apoptosis, decreasing cyclin E and bcl-2 while increasing p27 and bax.
Why it matters
This is one of the few studies testing purified inotodiol rather than a whole Chaga extract or a mixed triterpenoid fraction, so its cell cycle findings can be attributed to the compound itself.
The numbers in context
Dose-dependent proliferation inhibition at 24 h (r=0.9999, p<0.01); apoptosis rate rose with concentration and incubation time (r=1.0, p<0.01); protein expression changes p<0.05.
How the study worked
MTT assay for proliferation, flow cytometry for cell cycle and apoptosis, and immunocytochemistry for cyclin E, p27, bcl-2, and bax expression in HeLa cells.
Who was studied
Human cervical cancer HeLa cell line.
What this study cannot tell us
Single cancer cell line, in vitro only, no animal or human data, and no reported concentration range in the abstract.
Read the original research
Inotodiol inhabits proliferation and induces apoptosis through modulating expression of cyclinE, p27, bcl-2, and bax in human cervical cancer HeLa cells.
Asian Pacific journal of cancer prevention : APJCP, 15(7), 3195-9
Citation
Zhao, Li-Wei; Zhong, Xiu-Hong; Yang, Shu-Yan; Zhang, Yu-Zhen; Yang, Nai-Jun. (2014). Inotodiol inhabits proliferation and induces apoptosis through modulating expression of cyclinE, p27, bcl-2, and bax in human cervical cancer HeLa cells.. Asian Pacific journal of cancer prevention : APJCP, 15(7), 3195-9.